Tuning the structure and properties of cell-embedded gelatin hydrogels for tumor organoids

Tumor organoids have great potential as a 3D in vitro system to model cancer. In this work, we studied how the structure of hydrogels based on gelatin with methacryloyl groups (GeIMA) can affect their usage tumor organoids. To end, different levels methacrylation and cellulose nanocrystals (NCC) or reduced graphene oxide (rGO) were prepared used encapsulate human colon carcinoma cells (RKO). Mechanical properties measured dynamic conditions at 37°C water. Results showed that NCC was able provide higher mechanical stability hydrogels. RKO embedded GelMA proliferate within hydrogels, leading formation after 48 h. crosslink densities tended show cell population possibly due level rigidity displayed by these